Imaging activity of individual neurons in the living eyeA) GCaMP6s fluorescent retinal neurons in a WT mouse retina imaged in vivo using an adaptive optics ophthalmoscope focused at the ganglion cell layer. B) Color map showing normalized response amplitude to the UV (365 nm) stimulus. C) Color map of response phase to the 365 nm stimulus. D) Cell segmentation mask constructed from fluorescence intensity image (B) and response color maps (C & D). Scale bar indicates 10 µm and applies to A-D. E & F) Response time courses of cells indicated in A-D. Shaded bars indicate presentation of the 365 nm LED at 20 µW. Two consecutive trials are shown, the first at the top. Cell E shows ON responses to UV light. Cell F shows OFF responses to UV light. (Submitted manuscript to PNAS).
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Orientation selectivity in the primate lateral geniculate nucleusA) Orientation tuning curve of mean (F0) and fundamental harmonic (F1) responses. Gratings and arrows in A illustrate the stimuli presented on the monitor. B) Complex cell in primary visual cortex (V1). C) Orientation-selective cell (K-o cell). This cell shows “simple-like” behaviour with pronounced phase-locked F1 response. D) Second example of a K-o cell; this cell shows “complex-like” behaviour with increased average firing (F0) for preferred orientations.
PMID: 23595745 |
Direct connectivity of koniocellular Blue-ON cells in the lateral geniculate nucleus to primary visual cortexCollision test of a Blue-ON K cell. Consecutive recordings (n = 59) of a collision test for a Blue-ON K cell represented as a color map. Each row shows one stimulation trial aligned to the stimulus artifact. Two trials are plotted above in two-dimensional representation for reference. Stimulation delay began at 2 ms, decreased to 1.1 ms and increased back to 2 ms. Green asterisks indicate spontaneous spikes; thunderbolts, stimulus artifacts; and red arrows, antidromic spikes. The antidromic spikes in the color map appear as an interrupted blue line. The antidromic spike was not detected when the delay fell below 1.6 ms but reappeared when the delay increased above 1.5 ms.
PMID: 24703370 |